Aggie treeline field experiment data

sample summary

click to open

Soils were collected around trees on treelines in the western brooks range Alaska varying in soil moisture. Three sites were chosen: Tussock tundra (Mesic), Wet Sedge (Hydric), and Dryas-lichen tundra (Xeric). 8 similar trees were chosen per treatment based on their DBH and proximity to the treeline. Snow fences were constructed as a treatment to build larger snow packs around the given trees in winter and compared against a control group where nothing was done besides sampling. In order to reduce impact on these tree-plots 8 Ancillary trees were also chosen based on the same DBH, proximity to treeline parameters and used for seasonal sampling.
A previous project conducted at these sites fertilized soils around similar trees. These soils were also sampled to identify long term effects of fertilization. Soils were collected from control and snowfence trees in march and late may/early June (Just after thaw) along with resin strips (Except in 2020). Soil pore water was taken regularly during the growing season (2017-2019). During 2019 collection frequencies reduced due to staffing and were disrupted due to COVID in 2020 with an inability to visit the field sites.


Snowfence Vs. Control

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Extracts:

K2SO4

click to open
click to open ANOVA results
analyte Site YEAR TIME p.value asterisk
MBN Xeric 2018 Early Spring 0.0459993 *
PO4 Mesic 2017 Late Winter 0.0005795 *
PO4.fum Mesic 2017 Late Winter 0.0014443 *
TFPA Hydric 2018 Early Spring 0.0124263 *
TRS Xeric 2018 Early Spring 0.0015616 *

All comparisons

analyte Site YEAR TIME p.value asterisk
MBC Hydric 2017 Late Winter 0.9242528 NA
MBC Hydric 2018 Early Spring 0.6309733 NA
MBC Hydric 2019 Early Spring 0.1736374 NA
MBC Mesic 2017 Late Winter 0.2351039 NA
MBC Mesic 2018 Early Spring 0.3767047 NA
MBC Mesic 2019 Early Spring 0.4034522 NA
MBC Xeric 2017 Late Winter 0.8767061 NA
MBC Xeric 2018 Early Spring 0.0723087 NA
MBC Xeric 2019 Early Spring 0.5367112 NA
MBN Hydric 2017 Late Winter 0.4577312 NA
MBN Hydric 2018 Early Spring 0.5318262 NA
MBN Hydric 2019 Early Spring 0.1827473 NA
MBN Mesic 2017 Late Winter 0.4644693 NA
MBN Mesic 2018 Early Spring 0.4257308 NA
MBN Mesic 2019 Early Spring 0.5062197 NA
MBN Xeric 2017 Late Winter 0.2733834 NA
MBN Xeric 2018 Early Spring 0.0459993 *
MBN Xeric 2019 Early Spring 0.3603539 NA
NH4 Hydric 2017 Late Winter 0.4228383 NA
NH4 Hydric 2018 Early Spring 0.3189400 NA
NH4 Hydric 2019 Early Spring 0.2587825 NA
NH4 Mesic 2017 Late Winter 0.6445248 NA
NH4 Mesic 2018 Early Spring 0.2476791 NA
NH4 Mesic 2019 Early Spring 0.2868158 NA
NH4 Xeric 2017 Late Winter 0.1265287 NA
NH4 Xeric 2018 Early Spring 0.1018585 NA
NH4 Xeric 2019 Early Spring 0.3190037 NA
NO3 Hydric 2017 Late Winter 0.7447394 NA
NO3 Hydric 2018 Early Spring 0.1286781 NA
NO3 Hydric 2019 Early Spring 0.3457828 NA
NO3 Mesic 2017 Late Winter 0.4303936 NA
NO3 Mesic 2018 Early Spring 0.5627412 NA
NO3 Mesic 2019 Early Spring 0.2782300 NA
NO3 Xeric 2017 Late Winter 0.2067186 NA
NO3 Xeric 2018 Early Spring 0.5964418 NA
NO3 Xeric 2019 Early Spring 0.5464968 NA
PO4 Hydric 2017 Late Winter 0.6219360 NA
PO4 Hydric 2018 Early Spring 0.6332027 NA
PO4 Hydric 2019 Early Spring 0.9208946 NA
PO4 Mesic 2017 Late Winter 0.0005795 *
PO4 Mesic 2018 Early Spring 0.2821892 NA
PO4 Mesic 2019 Early Spring NaN NA
PO4 Xeric 2017 Late Winter 0.2969134 NA
PO4 Xeric 2018 Early Spring 0.1286896 NA
PO4 Xeric 2019 Early Spring 0.2288003 NA
PO4.fum Hydric 2017 Late Winter 0.3946057 NA
PO4.fum Hydric 2018 Early Spring 0.9225939 NA
PO4.fum Hydric 2019 Early Spring 0.8841150 NA
PO4.fum Mesic 2017 Late Winter 0.0014443 *
PO4.fum Mesic 2018 Early Spring 0.8352359 NA
PO4.fum Mesic 2019 Early Spring 0.7492556 NA
PO4.fum Xeric 2017 Late Winter 0.5309334 NA
PO4.fum Xeric 2018 Early Spring 0.3224832 NA
PO4.fum Xeric 2019 Early Spring 0.2023210 NA
TFPA Hydric 2017 Late Winter 0.5821164 NA
TFPA Hydric 2018 Early Spring 0.0124263 *
TFPA Hydric 2019 Early Spring 0.4173300 NA
TFPA Mesic 2017 Late Winter 0.0743117 NA
TFPA Mesic 2018 Early Spring 0.6880129 NA
TFPA Mesic 2019 Early Spring 0.3348522 NA
TFPA Xeric 2017 Late Winter 0.6976218 NA
TFPA Xeric 2018 Early Spring 0.4146803 NA
TFPA Xeric 2019 Early Spring 0.2464429 NA
TN.fum Hydric 2017 Late Winter 0.6137744 NA
TN.fum Hydric 2018 Early Spring 0.9945790 NA
TN.fum Hydric 2019 Early Spring 0.3533706 NA
TN.fum Mesic 2017 Late Winter 0.2196318 NA
TN.fum Mesic 2018 Early Spring 0.4382704 NA
TN.fum Mesic 2019 Early Spring 0.3518988 NA
TN.fum Xeric 2017 Late Winter 0.2101150 NA
TN.fum Xeric 2018 Early Spring 0.0976950 NA
TN.fum Xeric 2019 Early Spring 0.7061835 NA
TN.k2so4 Hydric 2017 Late Winter 0.4339124 NA
TN.k2so4 Hydric 2018 Early Spring 0.1324980 NA
TN.k2so4 Hydric 2019 Early Spring 0.4933220 NA
TN.k2so4 Mesic 2017 Late Winter 0.2813560 NA
TN.k2so4 Mesic 2018 Early Spring 0.5504293 NA
TN.k2so4 Mesic 2019 Early Spring 0.3198908 NA
TN.k2so4 Xeric 2017 Late Winter 0.1871344 NA
TN.k2so4 Xeric 2018 Early Spring 0.1557129 NA
TN.k2so4 Xeric 2019 Early Spring 0.3032183 NA
TOC.fum Hydric 2017 Late Winter 0.4751290 NA
TOC.fum Hydric 2018 Early Spring 0.5239418 NA
TOC.fum Hydric 2019 Early Spring 0.3488814 NA
TOC.fum Mesic 2017 Late Winter 0.0835000 NA
TOC.fum Mesic 2018 Early Spring 0.3804673 NA
TOC.fum Mesic 2019 Early Spring 0.3234169 NA
TOC.fum Xeric 2017 Late Winter 0.5379341 NA
TOC.fum Xeric 2018 Early Spring 0.1392725 NA
TOC.fum Xeric 2019 Early Spring 0.9413714 NA
TOC.k2so4 Hydric 2017 Late Winter 0.4555155 NA
TOC.k2so4 Hydric 2018 Early Spring 0.1300379 NA
TOC.k2so4 Hydric 2019 Early Spring 0.4229420 NA
TOC.k2so4 Mesic 2017 Late Winter 0.1194345 NA
TOC.k2so4 Mesic 2018 Early Spring 0.4628987 NA
TOC.k2so4 Mesic 2019 Early Spring 0.3520008 NA
TOC.k2so4 Xeric 2017 Late Winter 0.2140845 NA
TOC.k2so4 Xeric 2018 Early Spring 0.1504672 NA
TOC.k2so4 Xeric 2019 Early Spring 0.2028911 NA
TRS Hydric 2017 Late Winter 0.5977676 NA
TRS Hydric 2018 Early Spring 0.1616088 NA
TRS Hydric 2019 Early Spring 0.2283456 NA
TRS Mesic 2017 Late Winter 0.3575127 NA
TRS Mesic 2018 Early Spring 0.5151771 NA
TRS Mesic 2019 Early Spring 0.6518428 NA
TRS Xeric 2017 Late Winter 0.8898936 NA
TRS Xeric 2018 Early Spring 0.0015616 *
TRS Xeric 2019 Early Spring 0.4527488 NA
phenolics Hydric 2017 Late Winter 0.7061021 NA
phenolics Hydric 2018 Early Spring 0.1059705 NA
phenolics Hydric 2019 Early Spring 0.2626072 NA
phenolics Mesic 2017 Late Winter 0.5096703 NA
phenolics Mesic 2018 Early Spring 0.3014450 NA
phenolics Mesic 2019 Early Spring 0.0971096 NA
phenolics Xeric 2017 Late Winter 0.5109847 NA
phenolics Xeric 2018 Early Spring 0.8679635 NA
phenolics Xeric 2019 Early Spring 0.6036452 NA

Significant comparisons

H2O:

click to open
click to open ANOVA results
analyte Site YEAR TIME p.value asterisk
NH4.H2O Xeric 2018 Early Spring 0.0273894 *
TRS.H2O Xeric 2018 Early Spring 0.0254819 *
analyte Site YEAR TIME p.value asterisk
NH4.H2O Hydric 2018 Early Spring 0.2889907 NA
NH4.H2O Hydric 2019 Early Spring 0.5343913 NA
NH4.H2O Mesic 2018 Early Spring 0.5420167 NA
NH4.H2O Mesic 2019 Early Spring 0.1883625 NA
NH4.H2O Xeric 2018 Early Spring 0.0273894 *
NH4.H2O Xeric 2019 Early Spring 0.1494704 NA
NO3.H2O Hydric 2018 Early Spring 0.8209811 NA
NO3.H2O Hydric 2019 Early Spring 0.4171057 NA
NO3.H2O Mesic 2018 Early Spring 0.5466433 NA
NO3.H2O Mesic 2019 Early Spring 0.4726539 NA
NO3.H2O Xeric 2018 Early Spring 0.3951196 NA
NO3.H2O Xeric 2019 Early Spring 0.5685136 NA
PO4.H2O Hydric 2018 Early Spring 0.5219912 NA
PO4.H2O Hydric 2019 Early Spring 0.3982464 NA
PO4.H2O Mesic 2018 Early Spring 0.3220990 NA
PO4.H2O Mesic 2019 Early Spring 0.1862574 NA
PO4.H2O Xeric 2018 Early Spring 0.2169554 NA
PO4.H2O Xeric 2019 Early Spring 0.1464784 NA
TFPA.H2O Hydric 2018 Early Spring 0.2247949 NA
TFPA.H2O Hydric 2019 Early Spring 0.0668502 NA
TFPA.H2O Mesic 2018 Early Spring 0.7016381 NA
TFPA.H2O Mesic 2019 Early Spring 0.6210319 NA
TFPA.H2O Xeric 2018 Early Spring 0.8390267 NA
TFPA.H2O Xeric 2019 Early Spring 0.8229049 NA
TRS.H2O Hydric 2018 Early Spring 0.4725506 NA
TRS.H2O Hydric 2019 Early Spring 0.7770201 NA
TRS.H2O Mesic 2018 Early Spring 0.0997968 NA
TRS.H2O Mesic 2019 Early Spring 0.7744091 NA
TRS.H2O Xeric 2018 Early Spring 0.0254819 *
TRS.H2O Xeric 2019 Early Spring 0.2437419 NA

Pore water:

click to open
click to open
click to open LME results
analyte variable numDF denDF F-value p_value asterisk
NH4 MONTH 1 1533 28.033374 0.000 *
NH4 YEAR 1 1533 369.937357 0.000 *
NO3 MONTH 1 1533 4.621791 0.032 *
NO3 YEAR 1 1533 142.897963 0.000 *
NO3 Site 2 1533 12.405382 0.000 *
NO3 treatment 1 1533 14.671778 0.000 *
PO4 MONTH 1 1497 8.571502 0.003 *
PO4 YEAR 1 1497 463.374925 0.000 *
PO4 Site 2 1497 4.888577 0.008 *
TFPA Site 2 1463 9.385003 0.000 *
TRS MONTH 1 1540 41.938043 0.000 *
TRS YEAR 1 1540 69.095406 0.000 *
analyte variable numDF denDF F-value p_value asterisk
Mass MONTH 1 1463 0.0342764 0.853 NA
Mass YEAR 1 1463 1.5967304 0.207 NA
Mass Site 2 1463 1.0022801 0.367 NA
Mass treatment 1 1463 0.9551155 0.329 NA
NH4 MONTH 1 1533 28.0333740 0.000 *
NH4 YEAR 1 1533 369.9373565 0.000 *
NH4 Site 2 1533 0.9328648 0.394 NA
NH4 treatment 1 1533 2.7065628 0.100 NA
NO3 MONTH 1 1533 4.6217907 0.032 *
NO3 YEAR 1 1533 142.8979632 0.000 *
NO3 Site 2 1533 12.4053825 0.000 *
NO3 treatment 1 1533 14.6717780 0.000 *
PO4 MONTH 1 1497 8.5715020 0.003 *
PO4 YEAR 1 1497 463.3749246 0.000 *
PO4 Site 2 1497 4.8885771 0.008 *
PO4 treatment 1 1497 1.0705854 0.301 NA
TFPA MONTH 1 1463 0.6014594 0.438 NA
TFPA YEAR 1 1463 0.2499585 0.617 NA
TFPA Site 2 1463 9.3850031 0.000 *
TFPA treatment 1 1463 0.0000732 0.993 NA
TRS MONTH 1 1540 41.9380431 0.000 *
TRS YEAR 1 1540 69.0954058 0.000 *
TRS Site 2 1540 2.7695988 0.063 NA
TRS treatment 1 1540 1.0642374 0.302 NA

Resin strip:

click to open

click to open ANOVA results

Differences between sites.

Treatment differences were almost non existent.only over winter NH4 Xeric 2019 showed differences between snowfence and control.

analyte YEAR Treatment Purpose2 p.value asterisk
Ammonium 2017 Control OW 0.1828669 NA
Ammonium 2017 Snowfence OW 0.2359539 NA
Ammonium 2018 Control OW-GS 0.0730018 NA
Ammonium 2018 Control GS 0.0119577 *
Ammonium 2018 Snowfence OW-GS 0.0263591 *
Ammonium 2018 Snowfence GS 0.0000326 *
Ammonium 2019 Control OW 0.0143492 *
Ammonium 2019 Control GS 0.0436442 *
Ammonium 2019 Snowfence OW 0.0065189 *
Ammonium 2019 Snowfence GS 0.0040354 *
Ammonium 2020 Control OW-GS 0.1934751 NA
Ammonium 2020 Snowfence OW-GS 0.5398501 NA
Ammonium 2021 Control OW 0.7070097 NA
Ammonium 2021 Snowfence OW 0.3932623 NA
Nitrate 2017 Control OW 0.4052575 NA
Nitrate 2017 Snowfence OW 0.0538127 NA
Nitrate 2018 Control OW-GS 0.3414035 NA
Nitrate 2018 Control GS 0.2097259 NA
Nitrate 2018 Snowfence OW-GS 0.1415409 NA
Nitrate 2018 Snowfence GS 0.1967999 NA
Nitrate 2019 Control OW NaN NA
Nitrate 2019 Control GS 0.4086785 NA
Nitrate 2019 Snowfence OW NaN NA
Nitrate 2019 Snowfence GS 0.4388928 NA
Nitrate 2020 Control OW-GS 0.0892656 NA
Nitrate 2020 Snowfence OW-GS 0.0181929 *
Nitrate 2021 Control OW 0.0037983 *
Nitrate 2021 Snowfence OW 0.0000001 *
Phosphate 2017 Control OW 0.0000034 *
Phosphate 2017 Snowfence OW 0.0056395 *
Phosphate 2018 Control OW-GS 0.0000949 *
Phosphate 2018 Control GS 0.0033205 *
Phosphate 2018 Snowfence OW-GS 0.0510462 NA
Phosphate 2018 Snowfence GS 0.0535561 NA
Phosphate 2019 Control OW 0.0048510 *
Phosphate 2019 Control GS NaN NA
Phosphate 2019 Snowfence OW 0.0233137 *
Phosphate 2019 Snowfence GS NaN NA
Phosphate 2020 Control OW-GS 0.0133618 *
Phosphate 2020 Snowfence OW-GS 0.3372624 NA
Phosphate 2021 Control OW 0.0334202 *
Phosphate 2021 Snowfence OW 0.0336466 *

Comparing Sites (Significant only)

## NULL

Seasonal Extracts and pore water (Ancillary plots)

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Extracts:

Due to soil plot concerns only ancillary plots were harvested multiple times per year. K2SO4:

click to open

H2O:

click to open

Pore water:

click to open
click to open LME results
analyte variable numDF denDF F-value p_value asterisk
NH4 MONTH 1 2243 26.040128 0.000 *
NH4 YEAR 1 2243 286.445196 0.000 *
NO3 YEAR 1 2206 99.966287 0.000 *
NO3 Site 2 2206 34.799483 0.000 *
NO3 treatment 2 2206 5.281392 0.005 *
PO4 MONTH 1 2178 30.168079 0.000 *
PO4 YEAR 1 2178 379.187416 0.000 *
PO4 Site 2 2178 4.179465 0.015 *
TFPA MONTH 1 2130 4.696632 0.030 *
TFPA Site 2 2130 4.727536 0.009 *
TRS MONTH 1 2231 41.523906 0.000 *
TRS YEAR 1 2231 114.791131 0.000 *
TRS Site 2 2231 4.821214 0.008 *
TRS treatment 2 2231 6.195714 0.002 *
analyte variable numDF denDF F-value p_value asterisk
Mass MONTH 1 2208 0.0612205 0.805 NA
Mass YEAR 1 2208 1.8015229 0.180 NA
Mass Site 2 2208 1.2360672 0.291 NA
Mass treatment 2 2208 1.1057461 0.331 NA
NH4 MONTH 1 2243 26.0401282 0.000 *
NH4 YEAR 1 2243 286.4451963 0.000 *
NH4 Site 2 2243 0.1469776 0.863 NA
NH4 treatment 2 2243 1.3044999 0.272 NA
NO3 MONTH 1 2206 0.0094192 0.923 NA
NO3 YEAR 1 2206 99.9662866 0.000 *
NO3 Site 2 2206 34.7994825 0.000 *
NO3 treatment 2 2206 5.2813921 0.005 *
PO4 MONTH 1 2178 30.1680789 0.000 *
PO4 YEAR 1 2178 379.1874157 0.000 *
PO4 Site 2 2178 4.1794646 0.015 *
PO4 treatment 2 2178 1.3890078 0.250 NA
TFPA MONTH 1 2130 4.6966325 0.030 *
TFPA YEAR 1 2130 0.0016565 0.968 NA
TFPA Site 2 2130 4.7275359 0.009 *
TFPA treatment 2 2130 0.8810253 0.415 NA
TRS MONTH 1 2231 41.5239057 0.000 *
TRS YEAR 1 2231 114.7911306 0.000 *
TRS Site 2 2231 4.8212145 0.008 *
TRS treatment 2 2231 6.1957137 0.002 *

Resin strip:

click to open

Treeline project Extracts and pore water (Ancillary, Control, Snowfence plots)

click to open

Extracts:

Due to soil plot concerns only ancillary plots were harvested multiple times per year. K2SO4:

click to open

H2O:

click to open

Pore water:

click to open
click to open LME results

Resin strip:

click to open

Resin strips were extracted in 2M KCl

click to open

ANOVA results. Differences between sites


All Extracts and pore water (Ancillary, Control, Snowfence, and fertilized plots)

click to open

Extracts:

Due to soil plot concerns only ancillary plots were harvested multiple times per year. K2SO4:

click to open

H2O:

click to open

Pore water:

click to open
click to open LME results

Resin strip:

click to open

Resin strips were extracted in 2M KCl


Destructive lysimeter test

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Pore water:

click to open

Extractions

click to open


FTICR analysis

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Homogenized soil samples collected in the field in early(end of may begining of June) and late spring (Late June or early July) from 2017, 2018 and 2019 were sent to EMSL for FTICR analysis.

1. FTICR domains

click to open

2. Comparing polar vs. non-polar extracts

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Non-polar extracts were strongly dominated by low-O aliphatic molecules and skewed the overall trends when we combined results from polar and non-polar extracts. We therefore analyze the two extract types separately, and focus primarily on the polar (water) extracts.

3. Polar extracts

click to open
3.1. PERMANOVA

Polar:

click to open
Df SumsOfSqs MeanSqs F.Model R2 Pr(>F)
Site 2 0.2048378 0.1024189 109.2342208 0.7080634 0.001
Year 1 0.0011243 0.0011243 1.1990670 0.0038862 0.274
Season 2 0.0013712 0.0006856 0.7312319 0.0047399 0.493
Site:Year 2 0.0278093 0.0139047 14.8299373 0.0961286 0.001
Site:Season 3 0.0083759 0.0027920 2.9777402 0.0289529 0.034
Year:Season 1 0.0007694 0.0007694 0.8205613 0.0026595 0.388
Residuals 48 0.0450052 0.0009376 NA 0.1555696 NA
Total 59 0.2892930 NA NA 1.0000000 NA

Polar PERMANOVA results

Site, Site:Year were significant (p < 0.05)

Site accounted for 71 % of total variation among samples (R2= 0.71)

Non-Polar:

click to open
Df SumsOfSqs MeanSqs F.Model R2 Pr(>F)
Site 2 0.0815983 0.0407992 95.7669139 0.6687323 0.001
Year 1 0.0031369 0.0031369 7.3631567 0.0257082 0.016
Season 2 0.0019339 0.0009670 2.2697396 0.0158494 0.121
Site:Year 2 0.0123377 0.0061689 14.4800301 0.1011128 0.001
Site:Season 3 0.0025707 0.0008569 2.0113723 0.0210679 0.120
Year:Season 1 -0.0000074 -0.0000074 -0.0173272 -0.0000605 0.999
Residuals 48 0.0204492 0.0004260 NA 0.1675900 NA
Total 59 0.1220194 NA NA 1.0000000 NA

Non-Polar PERMANOVA results

3.2. PCA
click to open

There was a strong separation among the three Site/soil types. Hydric and Xeric soils were strongly dominated by aliphatic (simple) molecules, whereas Mesic soils were dominated by lignin-like, aromatic, and condensed aromatic (complex) molecules.

Hydric Only

3.3. Van Krevelen
click to open 3.3.1. All treatments
click to open

3.3.2. Unique peaks by site
click to open

Class Xeric Mesic Hydric
aliphatic 172 483 294
aromatic 32 135 22
condensed aromatic 10 65 17
unsaturated/lignin 122 703 47
3.3.3. Seasonality Mesic and Hydric
click to open


3. Non-Polar extracts

click to open
3.1. PERMANOVA

Non-Polar:

click to open
Df SumsOfSqs MeanSqs F.Model R2 Pr(>F)
Site 2 0.0815983 0.0407992 95.7669139 0.6687323 0.001
Year 1 0.0031369 0.0031369 7.3631567 0.0257082 0.016
Season 2 0.0019339 0.0009670 2.2697396 0.0158494 0.121
Site:Year 2 0.0123377 0.0061689 14.4800301 0.1011128 0.001
Site:Season 3 0.0025707 0.0008569 2.0113723 0.0210679 0.120
Year:Season 1 -0.0000074 -0.0000074 -0.0173272 -0.0000605 0.999
Residuals 48 0.0204492 0.0004260 NA 0.1675900 NA
Total 59 0.1220194 NA NA 1.0000000 NA

Non-Polar PERMANOVA results

3.2. PCA
click to open

3.3. Van Krevelen
click to open 3.3.1. All treatments
click to open

3.3.2. Unique peaks by site
click to open

Class Xeric Mesic Hydric
aliphatic 172 483 294
aromatic 32 135 22
condensed aromatic 10 65 17
unsaturated/lignin 122 703 47
3.3.3. Seasonality Mesic and Hydric
click to open


Session Info

Session Info

Date run: 2023-09-11

## R version 4.2.3 (2023-03-15 ucrt)
## Platform: x86_64-w64-mingw32/x64 (64-bit)
## Running under: Windows 10 x64 (build 19045)
## 
## Matrix products: default
## 
## locale:
## [1] LC_COLLATE=English_United States.utf8 
## [2] LC_CTYPE=English_United States.utf8   
## [3] LC_MONETARY=English_United States.utf8
## [4] LC_NUMERIC=C                          
## [5] LC_TIME=English_United States.utf8    
## 
## attached base packages:
## [1] grid      stats     graphics  grDevices utils     datasets  methods  
## [8] base     
## 
## other attached packages:
##  [1] cowplot_1.1.1     agricolae_1.3-6   doBy_4.6.17       ggpubr_0.6.0     
##  [5] pracma_2.4.2      reshape2_1.4.4    ggbreak_0.1.2     ggExtra_0.10.0   
##  [9] lubridate_1.9.2   forcats_1.0.0     stringr_1.5.0     dplyr_1.1.2      
## [13] purrr_1.0.1       readr_2.1.4       tidyr_1.3.0       tibble_3.2.1     
## [17] tidyverse_2.0.0   ggbiplot_0.55     scales_1.2.1      plyr_1.8.8       
## [21] ggplot2_3.4.1     vegan_2.6-4       lattice_0.20-45   permute_0.9-7    
## [25] tarchetypes_0.7.7 targets_1.2.0    
## 
## loaded via a namespace (and not attached):
##  [1] colorspace_2.1-0      ggsignif_0.6.4        ellipsis_0.3.2       
##  [4] fs_1.6.2              aplot_0.2.0           rstudioapi_0.15.0    
##  [7] farver_2.1.1          listenv_0.9.0         furrr_0.3.1          
## [10] Deriv_4.1.3           fansi_1.0.4           codetools_0.2-19     
## [13] splines_4.2.3         cachem_1.0.8          knitr_1.43           
## [16] broom_1.0.5           cluster_2.1.4         shiny_1.7.4.1        
## [19] compiler_4.2.3        backports_1.4.1       Matrix_1.6-0         
## [22] fastmap_1.1.1         cli_3.6.1             later_1.3.1          
## [25] htmltools_0.5.5       tools_4.2.3           igraph_1.5.0         
## [28] gtable_0.3.3          glue_1.6.2            Rcpp_1.0.11          
## [31] carData_3.0-5         vctrs_0.6.3           nlme_3.1-162         
## [34] xfun_0.39             globals_0.16.2        ps_1.7.5             
## [37] timechange_0.2.0      mime_0.12             miniUI_0.1.1.1       
## [40] lifecycle_1.0.3       rstatix_0.7.2         future_1.33.0        
## [43] MASS_7.3-58.2         microbenchmark_1.4.10 hms_1.1.3            
## [46] promises_1.2.0.1      parallel_4.2.3        yaml_2.3.7           
## [49] memoise_2.0.1         ggfun_0.1.2           yulab.utils_0.0.7    
## [52] labelled_2.12.0       stringi_1.7.12        highr_0.10           
## [55] klaR_1.7-2            AlgDesign_1.2.1       PNWColors_0.1.0      
## [58] rlang_1.1.1           pkgconfig_2.0.3       evaluate_0.21        
## [61] labeling_0.4.2        patchwork_1.1.2       processx_3.8.2       
## [64] tidyselect_1.2.0      parallelly_1.36.0     magrittr_2.0.3       
## [67] R6_2.5.1              generics_0.1.3        base64url_1.4        
## [70] combinat_0.0-8        pillar_1.9.0          haven_2.5.3          
## [73] withr_2.5.0           mgcv_1.8-42           abind_1.4-5          
## [76] car_3.1-2             questionr_0.7.8       utf8_1.2.3           
## [79] rmarkdown_2.23        tzdb_0.4.0            future.callr_0.8.1   
## [82] data.table_1.14.8     callr_3.7.3           digest_0.6.33        
## [85] xtable_1.8-4          httpuv_1.6.11         gridGraphics_0.5-1   
## [88] munsell_0.5.0         ggplotify_0.1.2