Aggie treeline field experiment data

Soils were collected around trees on treelines in the western brooks range Alaska varying in soil moisture. Three sites were chosen: Tussock tundra (Mesic), Wet Sedge (Hydric), and Dryas-lichen tundra (Xeric). 8 similar trees were chosen per treatment based on their DBH and proximity to the treeline. Snow fences were constructed as a treatment to build larger snow packs around the given trees in winter and compared against a control group where nothing was done besides sampling. In order to reduce impact on these tree-plots 8 Ancillary trees were also chosen based on the same DBH, proximity to treeline parameters and used for seasonal sampling.
A previous project conducted at these sites fertilized soils around similar trees. These soils were also sampled to identify long term effects of fertilization. Soils were collected from control and snowfence trees in march and late may/early June (Just after thaw) along with resin strips (Except in 2020). Soil pore water was taken regularly during the growing season (2017-2019). During 2019 collection frequencies reduced due to staffing and were disrupted due to COVID in 2020 with an inability to visit the field sites.

Extracts:

K2SO4

click to open

click to open ANOVA results

analyte	Site	YEAR	TIME	p.value	asterisk
MBN	Xeric	2018	Early Spring	0.0459993	*
PO4	Mesic	2017	Late Winter	0.0005795	*
PO4.fum	Mesic	2017	Late Winter	0.0014443	*
TFPA	Hydric	2018	Early Spring	0.0124263	*
TRS	Xeric	2018	Early Spring	0.0015616	*

All comparisons

analyte	Site	YEAR	TIME	p.value	asterisk
MBC	Hydric	2017	Late Winter	0.9242528	NA
MBC	Hydric	2018	Early Spring	0.6309733	NA
MBC	Hydric	2019	Early Spring	0.1736374	NA
MBC	Mesic	2017	Late Winter	0.2351039	NA
MBC	Mesic	2018	Early Spring	0.3767047	NA
MBC	Mesic	2019	Early Spring	0.4034522	NA
MBC	Xeric	2017	Late Winter	0.8767061	NA
MBC	Xeric	2018	Early Spring	0.0723087	NA
MBC	Xeric	2019	Early Spring	0.5367112	NA
MBN	Hydric	2017	Late Winter	0.4577312	NA
MBN	Hydric	2018	Early Spring	0.5318262	NA
MBN	Hydric	2019	Early Spring	0.1827473	NA
MBN	Mesic	2017	Late Winter	0.4644693	NA
MBN	Mesic	2018	Early Spring	0.4257308	NA
MBN	Mesic	2019	Early Spring	0.5062197	NA
MBN	Xeric	2017	Late Winter	0.2733834	NA
MBN	Xeric	2018	Early Spring	0.0459993	*
MBN	Xeric	2019	Early Spring	0.3603539	NA
NH4	Hydric	2017	Late Winter	0.4228383	NA
NH4	Hydric	2018	Early Spring	0.3189400	NA
NH4	Hydric	2019	Early Spring	0.2587825	NA
NH4	Mesic	2017	Late Winter	0.6445248	NA
NH4	Mesic	2018	Early Spring	0.2476791	NA
NH4	Mesic	2019	Early Spring	0.2868158	NA
NH4	Xeric	2017	Late Winter	0.1265287	NA
NH4	Xeric	2018	Early Spring	0.1018585	NA
NH4	Xeric	2019	Early Spring	0.3190037	NA
NO3	Hydric	2017	Late Winter	0.7447394	NA
NO3	Hydric	2018	Early Spring	0.1286781	NA
NO3	Hydric	2019	Early Spring	0.3457828	NA
NO3	Mesic	2017	Late Winter	0.4303936	NA
NO3	Mesic	2018	Early Spring	0.5627412	NA
NO3	Mesic	2019	Early Spring	0.2782300	NA
NO3	Xeric	2017	Late Winter	0.2067186	NA
NO3	Xeric	2018	Early Spring	0.5964418	NA
NO3	Xeric	2019	Early Spring	0.5464968	NA
PO4	Hydric	2017	Late Winter	0.6219360	NA
PO4	Hydric	2018	Early Spring	0.6332027	NA
PO4	Hydric	2019	Early Spring	0.9208946	NA
PO4	Mesic	2017	Late Winter	0.0005795	*
PO4	Mesic	2018	Early Spring	0.2821892	NA
PO4	Mesic	2019	Early Spring	NaN	NA
PO4	Xeric	2017	Late Winter	0.2969134	NA
PO4	Xeric	2018	Early Spring	0.1286896	NA
PO4	Xeric	2019	Early Spring	0.2288003	NA
PO4.fum	Hydric	2017	Late Winter	0.3946057	NA
PO4.fum	Hydric	2018	Early Spring	0.9225939	NA
PO4.fum	Hydric	2019	Early Spring	0.8841150	NA
PO4.fum	Mesic	2017	Late Winter	0.0014443	*
PO4.fum	Mesic	2018	Early Spring	0.8352359	NA
PO4.fum	Mesic	2019	Early Spring	0.7492556	NA
PO4.fum	Xeric	2017	Late Winter	0.5309334	NA
PO4.fum	Xeric	2018	Early Spring	0.3224832	NA
PO4.fum	Xeric	2019	Early Spring	0.2023210	NA
TFPA	Hydric	2017	Late Winter	0.5821164	NA
TFPA	Hydric	2018	Early Spring	0.0124263	*
TFPA	Hydric	2019	Early Spring	0.4173300	NA
TFPA	Mesic	2017	Late Winter	0.0743117	NA
TFPA	Mesic	2018	Early Spring	0.6880129	NA
TFPA	Mesic	2019	Early Spring	0.3348522	NA
TFPA	Xeric	2017	Late Winter	0.6976218	NA
TFPA	Xeric	2018	Early Spring	0.4146803	NA
TFPA	Xeric	2019	Early Spring	0.2464429	NA
TN.fum	Hydric	2017	Late Winter	0.6137744	NA
TN.fum	Hydric	2018	Early Spring	0.9945790	NA
TN.fum	Hydric	2019	Early Spring	0.3533706	NA
TN.fum	Mesic	2017	Late Winter	0.2196318	NA
TN.fum	Mesic	2018	Early Spring	0.4382704	NA
TN.fum	Mesic	2019	Early Spring	0.3518988	NA
TN.fum	Xeric	2017	Late Winter	0.2101150	NA
TN.fum	Xeric	2018	Early Spring	0.0976950	NA
TN.fum	Xeric	2019	Early Spring	0.7061835	NA
TN.k2so4	Hydric	2017	Late Winter	0.4339124	NA
TN.k2so4	Hydric	2018	Early Spring	0.1324980	NA
TN.k2so4	Hydric	2019	Early Spring	0.4933220	NA
TN.k2so4	Mesic	2017	Late Winter	0.2813560	NA
TN.k2so4	Mesic	2018	Early Spring	0.5504293	NA
TN.k2so4	Mesic	2019	Early Spring	0.3198908	NA
TN.k2so4	Xeric	2017	Late Winter	0.1871344	NA
TN.k2so4	Xeric	2018	Early Spring	0.1557129	NA
TN.k2so4	Xeric	2019	Early Spring	0.3032183	NA
TOC.fum	Hydric	2017	Late Winter	0.4751290	NA
TOC.fum	Hydric	2018	Early Spring	0.5239418	NA
TOC.fum	Hydric	2019	Early Spring	0.3488814	NA
TOC.fum	Mesic	2017	Late Winter	0.0835000	NA
TOC.fum	Mesic	2018	Early Spring	0.3804673	NA
TOC.fum	Mesic	2019	Early Spring	0.3234169	NA
TOC.fum	Xeric	2017	Late Winter	0.5379341	NA
TOC.fum	Xeric	2018	Early Spring	0.1392725	NA
TOC.fum	Xeric	2019	Early Spring	0.9413714	NA
TOC.k2so4	Hydric	2017	Late Winter	0.4555155	NA
TOC.k2so4	Hydric	2018	Early Spring	0.1300379	NA
TOC.k2so4	Hydric	2019	Early Spring	0.4229420	NA
TOC.k2so4	Mesic	2017	Late Winter	0.1194345	NA
TOC.k2so4	Mesic	2018	Early Spring	0.4628987	NA
TOC.k2so4	Mesic	2019	Early Spring	0.3520008	NA
TOC.k2so4	Xeric	2017	Late Winter	0.2140845	NA
TOC.k2so4	Xeric	2018	Early Spring	0.1504672	NA
TOC.k2so4	Xeric	2019	Early Spring	0.2028911	NA
TRS	Hydric	2017	Late Winter	0.5977676	NA
TRS	Hydric	2018	Early Spring	0.1616088	NA
TRS	Hydric	2019	Early Spring	0.2283456	NA
TRS	Mesic	2017	Late Winter	0.3575127	NA
TRS	Mesic	2018	Early Spring	0.5151771	NA
TRS	Mesic	2019	Early Spring	0.6518428	NA
TRS	Xeric	2017	Late Winter	0.8898936	NA
TRS	Xeric	2018	Early Spring	0.0015616	*
TRS	Xeric	2019	Early Spring	0.4527488	NA
phenolics	Hydric	2017	Late Winter	0.7061021	NA
phenolics	Hydric	2018	Early Spring	0.1059705	NA
phenolics	Hydric	2019	Early Spring	0.2626072	NA
phenolics	Mesic	2017	Late Winter	0.5096703	NA
phenolics	Mesic	2018	Early Spring	0.3014450	NA
phenolics	Mesic	2019	Early Spring	0.0971096	NA
phenolics	Xeric	2017	Late Winter	0.5109847	NA
phenolics	Xeric	2018	Early Spring	0.8679635	NA
phenolics	Xeric	2019	Early Spring	0.6036452	NA

Significant comparisons

H2O:

click to open

click to open ANOVA results

analyte	Site	YEAR	TIME	p.value	asterisk
NH4.H2O	Xeric	2018	Early Spring	0.0273894	*
TRS.H2O	Xeric	2018	Early Spring	0.0254819	*

analyte	Site	YEAR	TIME	p.value	asterisk
NH4.H2O	Hydric	2018	Early Spring	0.2889907	NA
NH4.H2O	Hydric	2019	Early Spring	0.5343913	NA
NH4.H2O	Mesic	2018	Early Spring	0.5420167	NA
NH4.H2O	Mesic	2019	Early Spring	0.1883625	NA
NH4.H2O	Xeric	2018	Early Spring	0.0273894	*
NH4.H2O	Xeric	2019	Early Spring	0.1494704	NA
NO3.H2O	Hydric	2018	Early Spring	0.8209811	NA
NO3.H2O	Hydric	2019	Early Spring	0.4171057	NA
NO3.H2O	Mesic	2018	Early Spring	0.5466433	NA
NO3.H2O	Mesic	2019	Early Spring	0.4726539	NA
NO3.H2O	Xeric	2018	Early Spring	0.3951196	NA
NO3.H2O	Xeric	2019	Early Spring	0.5685136	NA
PO4.H2O	Hydric	2018	Early Spring	0.5219912	NA
PO4.H2O	Hydric	2019	Early Spring	0.3982464	NA
PO4.H2O	Mesic	2018	Early Spring	0.3220990	NA
PO4.H2O	Mesic	2019	Early Spring	0.1862574	NA
PO4.H2O	Xeric	2018	Early Spring	0.2169554	NA
PO4.H2O	Xeric	2019	Early Spring	0.1464784	NA
TFPA.H2O	Hydric	2018	Early Spring	0.2247949	NA
TFPA.H2O	Hydric	2019	Early Spring	0.0668502	NA
TFPA.H2O	Mesic	2018	Early Spring	0.7016381	NA
TFPA.H2O	Mesic	2019	Early Spring	0.6210319	NA
TFPA.H2O	Xeric	2018	Early Spring	0.8390267	NA
TFPA.H2O	Xeric	2019	Early Spring	0.8229049	NA
TRS.H2O	Hydric	2018	Early Spring	0.4725506	NA
TRS.H2O	Hydric	2019	Early Spring	0.7770201	NA
TRS.H2O	Mesic	2018	Early Spring	0.0997968	NA
TRS.H2O	Mesic	2019	Early Spring	0.7744091	NA
TRS.H2O	Xeric	2018	Early Spring	0.0254819	*
TRS.H2O	Xeric	2019	Early Spring	0.2437419	NA

Pore water:

click to open

click to open

click to open LME results

analyte	variable	numDF	denDF	F-value	p_value	asterisk
NH4	MONTH	1	1533	28.033374	0.000	*
NH4	YEAR	1	1533	369.937357	0.000	*
NO3	MONTH	1	1533	4.621791	0.032	*
NO3	YEAR	1	1533	142.897963	0.000	*
NO3	Site	2	1533	12.405382	0.000	*
NO3	treatment	1	1533	14.671778	0.000	*
PO4	MONTH	1	1497	8.571502	0.003	*
PO4	YEAR	1	1497	463.374925	0.000	*
PO4	Site	2	1497	4.888577	0.008	*
TFPA	Site	2	1463	9.385003	0.000	*
TRS	MONTH	1	1540	41.938043	0.000	*
TRS	YEAR	1	1540	69.095406	0.000	*

analyte	variable	numDF	denDF	F-value	p_value	asterisk
Mass	MONTH	1	1463	0.0342764	0.853	NA
Mass	YEAR	1	1463	1.5967304	0.207	NA
Mass	Site	2	1463	1.0022801	0.367	NA
Mass	treatment	1	1463	0.9551155	0.329	NA
NH4	MONTH	1	1533	28.0333740	0.000	*
NH4	YEAR	1	1533	369.9373565	0.000	*
NH4	Site	2	1533	0.9328648	0.394	NA
NH4	treatment	1	1533	2.7065628	0.100	NA
NO3	MONTH	1	1533	4.6217907	0.032	*
NO3	YEAR	1	1533	142.8979632	0.000	*
NO3	Site	2	1533	12.4053825	0.000	*
NO3	treatment	1	1533	14.6717780	0.000	*
PO4	MONTH	1	1497	8.5715020	0.003	*
PO4	YEAR	1	1497	463.3749246	0.000	*
PO4	Site	2	1497	4.8885771	0.008	*
PO4	treatment	1	1497	1.0705854	0.301	NA
TFPA	MONTH	1	1463	0.6014594	0.438	NA
TFPA	YEAR	1	1463	0.2499585	0.617	NA
TFPA	Site	2	1463	9.3850031	0.000	*
TFPA	treatment	1	1463	0.0000732	0.993	NA
TRS	MONTH	1	1540	41.9380431	0.000	*
TRS	YEAR	1	1540	69.0954058	0.000	*
TRS	Site	2	1540	2.7695988	0.063	NA
TRS	treatment	1	1540	1.0642374	0.302	NA

Resin strip:

## NULL

Extracts:

Due to soil plot concerns only ancillary plots were harvested multiple times per year. K2SO4:

H2O:

Pore water:

click to open

click to open LME results

analyte	variable	numDF	denDF	F-value	p_value	asterisk
NH4	MONTH	1	2243	26.040128	0.000	*
NH4	YEAR	1	2243	286.445196	0.000	*
NO3	YEAR	1	2206	99.966287	0.000	*
NO3	Site	2	2206	34.799483	0.000	*
NO3	treatment	2	2206	5.281392	0.005	*
PO4	MONTH	1	2178	30.168079	0.000	*
PO4	YEAR	1	2178	379.187416	0.000	*
PO4	Site	2	2178	4.179465	0.015	*
TFPA	MONTH	1	2130	4.696632	0.030	*
TFPA	Site	2	2130	4.727536	0.009	*
TRS	MONTH	1	2231	41.523906	0.000	*
TRS	YEAR	1	2231	114.791131	0.000	*
TRS	Site	2	2231	4.821214	0.008	*
TRS	treatment	2	2231	6.195714	0.002	*

analyte	variable	numDF	denDF	F-value	p_value	asterisk
Mass	MONTH	1	2208	0.0612205	0.805	NA
Mass	YEAR	1	2208	1.8015229	0.180	NA
Mass	Site	2	2208	1.2360672	0.291	NA
Mass	treatment	2	2208	1.1057461	0.331	NA
NH4	MONTH	1	2243	26.0401282	0.000	*
NH4	YEAR	1	2243	286.4451963	0.000	*
NH4	Site	2	2243	0.1469776	0.863	NA
NH4	treatment	2	2243	1.3044999	0.272	NA
NO3	MONTH	1	2206	0.0094192	0.923	NA
NO3	YEAR	1	2206	99.9662866	0.000	*
NO3	Site	2	2206	34.7994825	0.000	*
NO3	treatment	2	2206	5.2813921	0.005	*
PO4	MONTH	1	2178	30.1680789	0.000	*
PO4	YEAR	1	2178	379.1874157	0.000	*
PO4	Site	2	2178	4.1794646	0.015	*
PO4	treatment	2	2178	1.3890078	0.250	NA
TFPA	MONTH	1	2130	4.6966325	0.030	*
TFPA	YEAR	1	2130	0.0016565	0.968	NA
TFPA	Site	2	2130	4.7275359	0.009	*
TFPA	treatment	2	2130	0.8810253	0.415	NA
TRS	MONTH	1	2231	41.5239057	0.000	*
TRS	YEAR	1	2231	114.7911306	0.000	*
TRS	Site	2	2231	4.8212145	0.008	*
TRS	treatment	2	2231	6.1957137	0.002	*

Resin strip:

click to open

Extracts:

Due to soil plot concerns only ancillary plots were harvested multiple times per year. K2SO4:

H2O:

Pore water:

click to open

click to open LME results

Resin strip:

Extracts:

Due to soil plot concerns only ancillary plots were harvested multiple times per year. K2SO4:

H2O:

Pore water:

click to open

click to open LME results

Resin strip:

Pore water:

Extractions

Homogenized soil samples collected in the field in early(end of may begining of June) and late spring (Late June or early July) from 2017, 2018 and 2019 were sent to EMSL for FTICR analysis.

1. FTICR domains

2. Comparing polar vs. non-polar extracts

3. Polar extracts

3.1. PERMANOVA

Non-Polar:

3.2. PCA

3.3. Van Krevelen

Date run: 2023-09-11

## R version 4.2.3 (2023-03-15 ucrt)
## Platform: x86_64-w64-mingw32/x64 (64-bit)
## Running under: Windows 10 x64 (build 19045)
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## [2] LC_CTYPE=English_United States.utf8   
## [3] LC_MONETARY=English_United States.utf8
## [4] LC_NUMERIC=C                          
## [5] LC_TIME=English_United States.utf8    
## 
## attached base packages:
## [1] grid      stats     graphics  grDevices utils     datasets  methods  
## [8] base     
## 
## other attached packages:
##  [1] cowplot_1.1.1     agricolae_1.3-6   doBy_4.6.17       ggpubr_0.6.0     
##  [5] pracma_2.4.2      reshape2_1.4.4    ggbreak_0.1.2     ggExtra_0.10.0   
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## [17] tidyverse_2.0.0   ggbiplot_0.55     scales_1.2.1      plyr_1.8.8       
## [21] ggplot2_3.4.1     vegan_2.6-4       lattice_0.20-45   permute_0.9-7    
## [25] tarchetypes_0.7.7 targets_1.2.0    
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##  [1] colorspace_2.1-0      ggsignif_0.6.4        ellipsis_0.3.2       
##  [4] fs_1.6.2              aplot_0.2.0           rstudioapi_0.15.0    
##  [7] farver_2.1.1          listenv_0.9.0         furrr_0.3.1          
## [10] Deriv_4.1.3           fansi_1.0.4           codetools_0.2-19     
## [13] splines_4.2.3         cachem_1.0.8          knitr_1.43           
## [16] broom_1.0.5           cluster_2.1.4         shiny_1.7.4.1        
## [19] compiler_4.2.3        backports_1.4.1       Matrix_1.6-0         
## [22] fastmap_1.1.1         cli_3.6.1             later_1.3.1          
## [25] htmltools_0.5.5       tools_4.2.3           igraph_1.5.0         
## [28] gtable_0.3.3          glue_1.6.2            Rcpp_1.0.11          
## [31] carData_3.0-5         vctrs_0.6.3           nlme_3.1-162         
## [34] xfun_0.39             globals_0.16.2        ps_1.7.5             
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## [40] lifecycle_1.0.3       rstatix_0.7.2         future_1.33.0        
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## [46] promises_1.2.0.1      parallel_4.2.3        yaml_2.3.7           
## [49] memoise_2.0.1         ggfun_0.1.2           yulab.utils_0.0.7    
## [52] labelled_2.12.0       stringi_1.7.12        highr_0.10           
## [55] klaR_1.7-2            AlgDesign_1.2.1       PNWColors_0.1.0      
## [58] rlang_1.1.1           pkgconfig_2.0.3       evaluate_0.21        
## [61] labeling_0.4.2        patchwork_1.1.2       processx_3.8.2       
## [64] tidyselect_1.2.0      parallelly_1.36.0     magrittr_2.0.3       
## [67] R6_2.5.1              generics_0.1.3        base64url_1.4        
## [70] combinat_0.0-8        pillar_1.9.0          haven_2.5.3          
## [73] withr_2.5.0           mgcv_1.8-42           abind_1.4-5          
## [76] car_3.1-2             questionr_0.7.8       utf8_1.2.3           
## [79] rmarkdown_2.23        tzdb_0.4.0            future.callr_0.8.1   
## [82] data.table_1.14.8     callr_3.7.3           digest_0.6.33        
## [85] xtable_1.8-4          httpuv_1.6.11         gridGraphics_0.5-1   
## [88] munsell_0.5.0         ggplotify_0.1.2